Evaluation of Oxidative Stress and some Biochemical Parameters in Normal Pregnant Women

Musher I. Kakey; Kamaran K. Abdoulrahman; Gulbahar R. Uthman

doi:10.14500/aro.10541

Musher I. Kakey Department of Chemistry, Faculty of Science and Health, Koya University, Koya KOY45, Kurdistan Region http://orcid.org/0000-0002-3702-4060
Kamaran K. Abdoulrahman Department of Chemistry, College of Science, Salahaddin University, Erbil, Kurdistan Region http://orcid.org/0000-0002-5982-2928
Gulbahar R. Uthman Department of Chemistry, College of Science, Salahaddin University, Erbil, Kurdistan Region http://orcid.org/0000-0001-5094-6644

Keywords: Pregnancy, Oxidative stress, Liver parameters, Lipid profiles, Kidney function

Abstract

Pregnancy is associated with many metabolic changes in normal pregnant woman, this leads to change in physiological, biochemical, and hematological parameters drastically. The test subjects were selected among those attending to Maternity Teaching Hospital in Erbil Governorate between March 2017 and August 2017. Four groups of individuals were included in this study, 230 pregnant women divided into three groups (Group 1 first trimester, Group 2 second trimester, and Group 3 third trimester) and Group 4 contained 90 nonpregnant women as control. Full automatic chemical analyzer (Cobas C311, Germany) was used to determine the biochemical parameters. The EL ×800 Absorbance Microplate Reader from BioTek (USA) instruments is used to measure the activity of superoxide dismutase (SOD), catalase (CAT), and malondialdehyde (MDA) in serum. The results of this study showed a significant increase in serum albumin, glucose, total bilirubin, direct bilirubin, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, creatinine, urea, cholesterol, low-density lipoprotein, and MDA in pregnant women as compared to the control group, also show significant decrease in high-density lipoprotein (HDL) level, SOD activity, and CAT activity in pregnant women as compared to nonpregnant women, and nonsignificant difference in uric acid, and HDL.

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Author Biographies

Musher I. Kakey, Department of Chemistry, Faculty of Science and Health, Koya University, Koya KOY45, Kurdistan Region

Musher is an assistant lecturer at the Department of Chemistry, Faculty of Science and Health, Koya University. He has received his B.Sc. in Chemistry at Koya University in 2010 and his MSc. at the University in 2014. He has 6 published articles.

Kamaran K. Abdoulrahman, Department of Chemistry, College of Science, Salahaddin University, Erbil, Kurdistan Region

Kamaran Kaiani Abdoulrahman is Assistant Professor at the Department of Chemistry of College of Science, Salahaddin University. He got his B.Sc. and M.Sc. degree in GKarl Franzens University Graz, Austria and the Ph. D. degree in Vienna University. His research interests are in Clinical Biochemistry and molecular biology.

Gulbahar R. Uthman, Department of Chemistry, College of Science, Salahaddin University, Erbil, Kurdistan Region

Gulbahar Rostam Uthman is Laboratory Assistant at Rezgary Hospital, Erbil. She got her B. Sc. degree in Chemistry in Salahaddin University in 2017. Her research interests are in Clinical Biochemistry. Gulbahar Rostam Uthman is a member Kurdistan Chemistry Sandicate.

References

Ali, D.M. 2015. Effect normal pregnancy and duration on liver enzymes tests. Global Journal of Medical Research, 15(1), pp.8-12.

Aziz, R., and Mahboob, T. 2007. Pre-eclampsia and lipid profile. Pakistan Journal of Medical Sciences, 23(5), pp.751.

Brizzi, P., Tonolo, G., Esposito, F., Puddu, L., Dessole, S., Maioli, M., and Milia, S. 1999. Lipoprotein metabolism during normal pregnancy. American Journal of Obstetrics and Gynecology, 181(2), pp.430-434.

Chaudhary, L., Tandon, O.P., Vaney, N., and Agarwal, N. Lipid peroxidation and antioxidant enzymes in gestational diabetics. Indian Journal of Physiology and Pharmacology, 47(4), pp.441-446.

Cunningham, F.G. 2005. Maternal physiology. Williams Obstetrics, 2005, pp.121-150.

Duraiswamy, S., Sheffield, J.S., Mcintire, D., Leveno, K., and Mayo, M.J. 2017. Updated etiology and significance of elevated bilirubin during pregnancy: changes parallel shift in demographics and vaccination status. Digestive Diseases and Sciences, 62(2), pp.517-525.

Erişir, M., Benzer, F., and Kandemir, F.M. 2009. Changes in the rate of lipid peroxidation in plasma and selected blood antioxidants before and during pregnancy in ewes. Acta Veterinaria Brno, 78(2), pp.237-242.

Festus, O.O., Idonije, O.B., Eseigbe, M.A., Okhiai, O., Unuabonah, F., and Dike, M. 2011. Comparative study of lipid profile of normal pregnant women in the different trimesters. Archives of Applied Science Research, 3(3), pp.528-532.

Gohel, M.G., Joshi, A.G., Anand, J.S., Makadia, J.S., and Kamariya, C.P. 2016. Evaluation of changes in liver function test in first, second and third trimester of normal pregnancy. International Journal of Reproduction, Contraception, Obstetrics and Gynecology, 2(4), pp.616-20.

González, I.M., Armada, E.R., Díaz, J.R., García, P.D., García, M.M., González, A.G., Fernández, C.B., Iñiguez, A.R., and Rayo, I.L. 2000. Practice guidelines of the spanish society of cardiology for the management of cardiac disease in pregnancy. Revista Espanola de Cardiologia, 53(11), pp.1474-95.

Hay, J.E. 2008. Liver disease in pregnancy. Hepatology, 47(3), pp.1067-1076.

Herrera, E. 2002. Lipid metabolism in pregnancy and its consequences in the fetus and newborn. Endocrine, 19(1), 43-55.

Kamili, A., Bengoumi, M., Oukessou, M., Faye, B., and Lefebvre, H.P. 2013. Assessment of glomerular filtration rate in normally hydrated and dehydrated dromedary camel by plasma exogenous creatinine clearance test. Emirates Journal of Food and Agriculture, 25(4), pp.314-319.

Loganathan, G., George, R., Eapen, C.E., Mathai, M., Jasper, P., Seshadri, L., Shankar, V., Paul, S., Joseph, G., Balasubramanian, K.A., and Chandy, G.M. 2005. Liver function tests in normal pregnancy: A study from Southern India. Indian Journal of Gastroenterology: Official Journal of the Indian Society of Gastroenterology, 24(6), pp.268-269.

Merabishvili, N.V., Kamladze, S.O., and Sulaberidze, G,T. 2006. Peculiarities of lipid metabolism during pregnancy. Georgian Medical News, 138, pp.86-89.

Miao, L., and Clair, D.K. 2009. Regulation of superoxide dismutase genes: Implications in disease. Free Radical Biology and Medicine, 47(4), pp.344-56.

Ogura, K., Miyatake, T., Fukui, O., Nakamura, T., Kameda, T., and Yoshino, G. 2002. Low-density lipoprotein particle diameter in normal pregnancy and preeclampsia. Journal of Atherosclerosis and Thrombosis, 9(1), pp.42-7.

Parchwani, D., and Patel, D. 2011. Status of lipid profile in pregnancy. National Journal of Medical Research, 1(1), pp.10-12.

Patel, S.S., Molnar, M.Z., Tayek, J.A., Ix, J.H., Noori, N., Benner, D., Heymsfield, S., Kopple, J.D., Kovesdy, C.P., and Kalantar-Zadeh, K. 2013. Serum creatinine as a marker of muscle mass in chronic kidney disease: Results of a cross- sectional study and review of literature. Journal of Cachexia, Sarcopenia and Muscle, 4(1), pp.19-29.

Pirzado, Z.A., Sngi, S.A., and Malik, R. 1999. High density lipoprotein cholesterol (HDL) metabolism and its role in ischemic heart disease. Pakistan Journal of Medical Research, 38(1), pp.38-41.

Saikumar, P., Jaya, D., and Devi, M.R. 2013. Oxidative stress in pregnancy. ISOR Journal of Dental and Medical Sciences, 3, pp.12-13.

Welty, F.K. 2013. How do elevated triglycerides and low HDL-cholesterol affect inflammation and atherothrombosis? Current Cardiology Reports, 15(9), pp.400.

Westbrook, R.H., Dusheiko, G., and Williamson, C. 2016. Pregnancy and liver disease. Journal of Hepatology, 64(4), pp.933-945.

Woollett, L.A. 2011. Transport of maternal cholesterol to the fetal circulation. Placenta, 1(32), pp.218-221.