The Role of Immune Defense in Serratia marcescens Nosocomial Infections

Ihsan A. Raheem; Fatima R. Abdul; Hanan T. Subhi

doi:10.14500/aro.11819

Ihsan A. Raheem Department of Medical Lab, College of Medical Techniques, Al-Farahidi University, Baghdad, – F.R. Iraq https://orcid.org/0000-0002-2097-027X
Fatima R. Abdul Department of Biology, College of Science, Mustansiriyah University, Baghdad – F.R. Iraq https://orcid.org/0000-0001-7590-4221
Hanan T. Subhi Department of Biology, Faculty of Science and Health, Koya University, Koya KOY45, Kurdistan Region – F.R. Iraq https://orcid.org/0000-0002-2432-7724

Keywords: Antibiotics susceptibility test, Interleukin-12, Neutrophil, Phagocytosis, Serratia marcescens, Virulence factors

Abstract

Developing resistance mechanisms leads to various nosocomial infections caused by opportunistic bacteria. Serratia marcescens are well known to be opportunistic and are equipped with an armory of virulence factors against host immune response. The study aims to detect the immune defense in patients infected with multidrug-resistant S. marcescens. The study includes 132 clinical samples, including burn, wound, otitis media, and urinary tract infection (UTI) at several hospitals in Baghdad, Iraq. All isolates are identified by cultivation on MacConkey agar, nutrient agar, and blood agar, followed by biochemical tests and assessment with the VITEK 2 compact system. The isolates are tested for antibiotic susceptibility tests, interleukin-12 (IL12) levels, neutrophil ability to phagocytosis, and complement C3 and C4 levels. Out of 120 positive cultures, six isolates are identified as S. marcescens. The urine samples are the most isolated source and a higher level of antibiotic resistance was noticed in ampicillin and cefotaxime (100%), whereas a lower level is in imipenem. Stimulation (p ꞊ 0.005) provided a significant increase in IL-12 production. The infection with the S. marcescens stimulated the neutrophil’s phagocytosis process compared with the control. The interplay role of virulence factors in S. marcescens influences its pathogenesis, antibiotic resistance, and immune response, particularly involving neutrophils and IL-12. Understanding these interactions is crucial for developing effective therapeutic strategies.

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Author Biographies

Ihsan A. Raheem, Department of Medical Lab, College of Medical Techniques, Al-Farahidi University, Baghdad, – F.R. Iraq

Ihsan A. Raheem is a lecturer at the College of Health and Medical Technologies, Al-Farahidi University. H got the B.Sc. degree in biology, the M.Sc. degree in microbiology, and the Ph.D. degree in microbiology. His research interests are in bacteriology.

Fatima R. Abdul , Department of Biology, College of Science, Mustansiriyah University, Baghdad – F.R. Iraq

Fatima R. Abdulis is a Professor at the Department of Biology, College of Science, Mustansiriyah University. She got the B.Sc. degree in biology, the M.Sc. degree in microbiology, and the Ph.D. degree in microbiology. Her research interests are in bacteria, infections, and immunity.

Hanan T. Subhi, Department of Biology, Faculty of Science and Health, Koya University, Koya KOY45, Kurdistan Region – F.R. Iraq

Hanan T. Subhi is an Assistant Prof. at the Department of Biology, Faculty of Science and Health, Koya University. She got the B.Sc. degree in biology, the M.Sc. degree in microbiology, and the Ph.D. degree in microbiology. Her research interests are in bacteriology and nanotechnology.

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The Role of Immune Defense in Serratia marcescens Nosocomial Infections

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