L-Tryptophan as Fluorescent Probe for Determination of Folic Acid in Some Pharmaceutical Products
A new fluorescent probe L-Tryptophan was reported for the determination of folic acid (FA), based on its quenching effect of the fluorescence intensity of L-Tryptophan. The concentration of folic acid was proportional to the quenched fluorescence intensity of L-Tryptophan at an emission wavelength of 365 nm in Britton– Robinson (BR) buffer solution of pH 7. Optimized conditions of pH, time, order of addition of the reagent, potential interferences, concentrations of L-Tryptophan and buffer were investigated. Folic acid was determined in a linear range of 2.0 to 16.0 μg ml-1 with a correlation coefficient R2 0.9974. The limits of detection LOD and quantification LOQ values were 0.09 μg ml-1 and 0.27 μg ml-1, respectively. The standard deviation (RSD) values for five replicated measurements of 2, 8, 16 μg ml-1 folic acid were between 0.23 % and 1.07%. This method is efficient for routine analysis and quality control assay as it is relatively interferences free.
Abd Ali, L.I., Qader, A.F., Salih, M.I., and Aboul-Enein, H.Y., 2019. Sensitive spectrofluorometric method for the determination of ascorbic acid in pharmaceutical nutritional supplements using acriflavine as a fluorescence reagent. Luminescence, 34(2), pp.168-174.
Al-Araji, R.R., Mashkour, M.S., and Jaffar Al-Mulla, E.A., 2017. Spectrophotometric determination of vitamin folic acid B9 in some drugs using 1, 2-naphthoquine-4-sulphonate (NQS). Nano Biomedicine and Engineering, 9(3), pp.208-213.
Aurora-Prado, M.S., Silva, C.A., Tavares, M.F., and Altria, K.D., 2004. Determination of folic acid in tablets by microemulsion electrokinetic chromatography. Journal of Chromatography A, 1051(1), pp.291-296.
Bailey, L.B., 2000. New standard for dietary folate intake in pregnant women. The American Journal of Clinical Nutrition, 71(5), pp.1304-1307.
Bhattacharyya, M., Chaudhuri, U., Poddar, R.K., 1990. Evidence for cooperative binding of CPZ with hemoglobin. Biochemical and Biophysical Research Communications, 167, pp.1146-1153.
Catharino, R.R., Godoy, H.T., and Lima-Pallone, J.A., 2006. Analytical methodology for folate and folic acid determination in food. Química Nova, 29(5), pp.970-972.
Chaudhary, A., Wang, J., and Prabhu, S., 2010. Development and validation of a high-performance liquid chromatography method for the simultaneous determination of aspirin and folic acid from nano-particulate systems. Biomedical Chromatography, 24(9), pp.919-925.
Crane, N.T., Wilson, D.B., Cook, D.A., Lewis, C.J., Yetley, E.A., and Rader, J.I., 1995. Evaluating food fortification options: General principles revisited with folic acid. American Journal of Public Health, 85(5), pp.660-666.
Cruces, B.C., Segura, C.A., Fernández, G.A., and Román, C.M., 1994. Fluorometric determination of folic acid based on its reaction with the fluorogenic reagent fluorescamine. Analytical Letters, 27(7), pp.1339-1353.
Czeize, A.E., and Dudas, I., 1992. Prevention of the first occurrence of neural tube defects by perioconceptional vitamin supplementation. The New England Journal of Medicine, 327(226), pp.1832-1835.
Deconinck, E., Crevits, S., Baten, P., Courselle, P., and De Beer, J., 2011. A validated ultra-high pressure liquid chromatographic method for qualification and quantification of folic acid in pharmaceutical preparations. Journal of Pharmaceutical and Biomedical Analysis, 54(5), pp.995-1000.
El-Leithy, E.S., Abdel-Bar, H.M., and El-Moneum, R.A., 2018. Validation of high performance liquid chromatographic method for folic acid assay. International Journal of Pharmaceutical Science Invention, 7(1), pp.1-5.
Ensafi, A.A., and Karimi, M.H., 2010. Modified multiwall carbon nanotubes paste electrode as a sensor for simultaneous determination of 6-thioguanine and folic acid using ferrocenedicarboxylic acid as a mediator. Journal of Electroanalytical Chemistry, 640(1), pp.75-83.
Flores, J.R., Penalvo, G.C., Mansilla, A.E., and Gomez, M.J., 2005. Capillary electrophoretic determination of methotrexate, leucovorin and folic acid in human urine. Journal of Chromatography B, 819(1), pp.141-147.
Kennedy, D., 2016. B vitamins and the brain: Mechanisms, dose and efficacy a review. Nutrients, 8(2), p.68.
Krishnaswamy, K., and Nair, K.M., 2001. Importance of folate in human nutrition. British Journal of Nutrition, 85(2), pp.115-124.
Lakowicz, J.R., 2006. Principles of Fluorescence Spectroscopy. 3rd ed. New York: Springer. pp.277-330.
Manzoori, J.L., Jouyban, A., Amjadi, M., and Soleymani, J., 2011. Spectrofluorimetric determination of folic acid in tablets and urine samples using 1, 10-phenanthroline-terbium probe. Luminescence, 26, pp.106-111.
Mirmoghtadaie, L., Ensafi, A.A., Kadivar, M., Shahedi, M., and Ganjali, M.R., 2013. Highly selective, sensitive and fast determination of folic acid in food samples using new electrodeposited gold nanoparticles by differential pulse voltammetry. International Journal of Electrochemical Science, 8, pp.3755-3767.
Moura, J.I., and Moita, G.C., 2012. Simultaneous determination of olanzapine and fluoxetine hydrochloride in pharmaceutical formulations by derivative spectrophotometry. Química Nova, 35(3), pp.627-633.
Nagaraja, P., Vasantha, R.A., and Yathirajan, H.S., 2002. Spectrophotometric determination of folic acid in pharmaceutical preparations by coupling reactions with iminodibenzyl or 3-aminophenol or sodium molybdate pyrocatechol. Analytical Biochemistry, 307(2), pp.316-321.
Nasser, C., Nobre, C., Mesquita, S., Ruiz, J.G., Carlos, H.R., Prouvot, L., and Yacubian, M.T., 2005. Natural variation of folate in cowpea seeds. Journal of Epilepsy and Clinical Neurophysiology, 11(4), pp.199-203.
Nelson, B.C., Sharpless, K.E., and Sander, L.C., 2006. Quantitative determination of folic acid in multivitamin/multielement tablets using liquid chromatography/ tandem mass spectrometry. Journal of Chromatography A, 1135(2), pp.203-211.
Nie, F., He, Y., and LU, J., 2000. An investigation of the chemiluminescence reaction in the sodium hypochlorite-folic acid-semicarbazide hydrochloride system. Microchemical Journal, 65(3), pp.319-323.
Oakley, G.P., Erickson, J.D., and Adams, M.J., 1995. Urgent need to increase folic acid consumption. JAMA, 274(21), pp.1717-1718.
Pacheco, S.S., Braga, C., Souza, A.I.D., and Figueiro, J.N., 2009. Effects of folic acid fortification on the prevalence of neural tube defects. Revista de Saúde Pública, 43(4), pp.565-571.
Pesce, M.A., and Bodourian, S.H., 1986. Evaluation of a fluorescence polarization immunoassay procedure for quantitation of methotrexate. Therapeutic Drug Monitoring, 8(1), p.115.
Póo-Prieto, R., Haytowitz, D.B., Holden, J.M., Rogers, G., Choumenkovitch, S.F., Jacques, P.F., and Selhub, J., 2006. Use of the affinity/HPLC method for quantitative estimation of folic acid in enriched cereal-grain products. The Journal of Nutrition, 136(12), pp.3079-3083.
Prasad, B.B., Tiwari, M.P., Madhuri, R., and Sharma, P.S., 2010. Development of a highly sensitive and selective hyphenated technique (molecularly imprinted micro-solid phase extraction fiber-molecularly imprinted polymer fiber sensor) for ultratrace analysis of folic acid. Analytica Chimica Acta, 662(1), pp.14-22.
Rao, G.R., Kanjilal, G., and Mohan, K.R., 1978. Extended application of folin-ciocalteu reagent in the determination of drugs. Analyst, 103(1230), pp.993-994.
Skoog, D.A., West, D.M., Holler, F.J., and Crouch, S.R., 2013. Fundamentals of Analytical Chemistry. 9th ed. Cengage Learning, Boston.
Vaze, V.D., and Srivastava, A.K., 2007. Electrochemical behavior of folic acid at calixarene based chemically modified electrodes and its determination by adsorptive stripping voltammetry. Electrochimica Acta, 53(4), pp.1713-1721.
Zare, H.R., Shishehbore, M.R., and Nematollahi, D., 2011. A highly sensitive and selective sensor on the basis of 4-hydroxy-2-(triphenylphosphonio) phenolate and multi-wall carbon nanotubes for electrocatalytic determination of folic acid in presence of ascorbic acid and uric acid. Electrochimica Acta, 58, pp.654-661.
Zhao, S., Yuan, H., Xie, C., and Xiao, D., 2006. Determination of folic acid by capillary electrophoresis with chemiluminescence detection. Journal of Chromatography A, 1107(1), pp. 290-293.
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