Effect of Static Magnetic Field on Bone Marrow Cellular Density
This study was undertaken to investigate the influence of static magnetic field (SMF) on bone marrow cellular density (BMCD) variation proportionally to bone trabeculae. Female albino Wistar rats exposed with 2.4 ± 0.2 millitesla for 1–4weeks duration continuously versus 1h, 2h, 6h, and 8h/day. Trephine biopsy of femurs bone was examined under optical microscope. Data analyzed with ImageJ software. Results showed that short time exposure per day did not enhance the BMCD compare to high exposure period/day. Six hours/day exposure during 1week increased the marrow cellular density (hypercellularity) significantly (P ≤ 0.05) compares to bone trabeculae. Contrarily, 8h/day exposure reduced the BMCD slightly and significantly (hypocellularity, about 50% reduction) due to 1week and 4weeks exposure duration, respectively. The SMF has associated bone marrow cellularity tendency of rat’s femur.
Amara, S., Abdelmelek, H., Salem, M.B., Abidi, R. and Sakly, M., 2006. Effects of static magnetic field exposure on hematological and biochemical parameters in rats. Brazilian Archives of Biology and Technology, 49, pp.889-895.
Amin, H.D., Brady, M.A., St-Pierre, J.P., Stevens, M.M., Overby, D.R. and Ethier, C.R., 2014. Stimulation of chondrogenic differentiation of adult human bone marrow-derived stromal cells by a moderate-strength static magnetic field. Tissue Engineering Part A, 20, pp.1612-1620.
Berg, B.C.V., Malghem, J., Lecouvet, F.E. and Maldague, B., 1998. Magnetic resonance imaging of the normal bone marrow. Skeletal Radiology, 27, pp.471-483.
Cakir, D.U., Yokus, B., Akdag, M.Z., Sert, C. and Mete, N., 2009. Alterations of hematological variations in rats exposed to extremely low frequency magnetic fields (50 Hz). Archives of Medical Research, 40, pp.352-356.
Cetin, N., Bilgili, A. and Eraslan, G., 2006. Effects of pulsed magnetic field chronic exposure on some hematological parameters in mice. Revue Medicine Veterinaire, 157, pp.68-71.
Chater, S., Abdelmelek, H., Pequignot, J.M., Sakly, M. and Rhouma, K.B., 2006. Effects of sub-acute exposure to static magnetic field on hematologic and biochemical parameters in pregnant rats. Electromagnetic Biology and Medicine, 25, pp.135-144.
Chen, G., Zhuo, Y., Tao, B., Liu, Q., Shang, W., Li, Y., Wang, Y., Li, Y., Zhang, L. and Fang, Y., 2020. Moderate SMFs attenuate bone loss in mice by promoting directional osteogenic differentiation of BMSCs. Stem Cell Research and Therapy, 11, pp.1-14.
Ciejka, E., Kleniewska, P., Skibska, B. and Goraca, A., 2011. Effects of extremely low frequency magnetic field on oxidative balance in brain of rats. Journal of Physiology and Pharmacology, 62, pp.657.
Djordjevich, D.M., de Luka, S.R., Milovanovich, I.D., Janković, S., Stefanović, S., Vesković-Moračanin, S., Ćirković, S., Ilić, A.Ž., Ristić- Djurović, J.L. and Trbovich, A.M., 2012. Hematological parameters’ changes in mice subchronically exposed to static magnetic fields of different orientations. Ecotoxicology and Environmental Safety, 81, pp.98-105.
Jouni, F.J., Abdolmaleki, P. and Movahedin, M., 2013. Investigation on the effect of static magnetic field up to 15 mT on the viability and proliferation rate of rat bone marrow stem cells. In Vitro Cellular and Developmental Biology-Animal, 49, pp.212-219.
Kim, E.C., Leesungbok, R., Lee, S.W., Lee, H.W., Park, S.H., Mah, S.J. and Ahn, S.J., 2015. Effects of moderate intensity static magnetic fields on human bone marrow‐derived mesenchymal stem cells. Bioelectromagnetics, 36, pp.267-276.
Lee, S.H., Erber, W., Porwit, A., Tomonaga, M., Peterson, L. and Hematology, I.C.S., 2008. ICSH guidelines for the standardization of bone marrow specimens and reports. International Journal of Laboratory Hematology, 30, pp.349-364.
Mustafa, B.T., Yab, S.P. and Ismail, A.H., 2020a. Impacts of materials on the intensity of uniform static magnetic fields using a multi Helmholtz coils design. In: AIP Conference Proceedings. AIP Publishing LLC, 020063.
Mustafa, B.T., Yaba, S. and Ismail, A.H., 2020b. Impacts of In Vivo Exposure Static Magnetic Field to Blood Viscosity. In: Materials Science Forum, Trans Tech Publications Ltd., pp.369-378.
Mustafa, B.T., Yaba, S.P. and Ismail, A.H., 2020c. Experimental Evaluation of the Static Magnetic Field Effect on White Blood Cells: In Vivo Study. In: Materials Science Forum. Trans Tech Publications Ltd., pp.412-419.
Mustafa, B.T., Yaba, S.P. and Ismail, A.H., 2020d. Influence of the static magnetic field on red blood cells parameters and platelets using tests of CBC and microscopy images. Biomedical Physics and Engineering Express, 6, p.025004.
Mustafa, B.T., Yaba, S.P. and Ismail, A.H., 2020e. Moderate range static magnetic field promoted variation of blood parameters: An in vitro study. ARO The Scientific Journal of Koya University, 8, pp.55-64.
Pedersen, L.M., Jarner, D. and Winge, J., 1993. Bone-marrow biopsy of the iliac bone followed by severe retroperitoneal hemorrhage. European Journal of Haematology, 51, pp.52-52.
Pirkhider Yaba, S. and Ismail, A.H., 2019. A review of the effects of magnetic field on main blood cells: In vivo and in vitro experiments. ZANCO Journal of Pure and Applied Sciences, 31, pp.40-50.
Sarvestani, A.S., Abdolmaleki, P., Mowla, S.J., Ghanati, F., Heshmati, E., Tavasoli, Z. and Jahromi, A.M., 2010. Static magnetic fields aggravate the effects of ionizing radiation on cell cycle progression in bone marrow stem cells. Micron, 41, pp.101-104.
Sihem, C., Hafedh, A., Mohsen, S., Marc, P.J. and Khmais, B.R., 2006. Effects of sub-acute exposure to magnetic field on blood hematological and biochemical parameters in female rats. The Turkish Journal of Hematology, 23, pp.182-187.
Sun, L.Y., Hsieh, D.K., Yu, T.C., Chiu, H.T., Lu, S.F., Luo, G.H., Kuo, T.K., Lee, O.K. and Chiou, T.W., 2009. Effect of pulsed electromagnetic field on the proliferation and differentiation potential of human bone marrow mesenchymal stem cells. Bioelectromagnetics: Journal of the Bioelectromagnetics Society, The Society for Physical Regulation in Biology and Medicine, The European Bioelectromagnetics Association, 30, pp.251-260.
Suvarna, K.S., Layton, C. and Bancroft, J.D. 2018. Bancroft’s Theory and Practice of Histological Techniques E-Book, Elsevier Health Sciences, Amsterdam, Netherlands.
Vergallo, C. and Dini, L., 2018. Comparative analysis of biological effects induced on different cell types by magnetic fields with magnetic flux densities in the range of 1-60 mT and frequencies up to 50 Hz. Sustainability, 10, pp.2776.
Xu, S., Okano, H., Tomita, N. and Ikada, Y., 2010. Recovery effects of a 180 mT static magnetic field on bone mineral density of osteoporotic lumbar vertebrae in ovariectomized rats. Evidence Based Complementary and Alternative Medicine, 2011, 620984.
Yan, J., Dong, L., Zhang, B. and Qi, N., 2010. Effects of extremely low-frequency magnetic field on growth and differentiation of human mesenchymal stem cells. Electromagnetic Biology and Medicine, 29, pp.165-176.
Yan, Q., Tomita, N. and Ikada, Y., 1998. Effects of static magnetic field on bone formation of rat femurs. Medical Engineering and Physics, 20, pp.397-402.
Yong, L., 1992. The theory and practice of histological techniques. Pathology, 24, p.320.
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