Molecular Typing of Human Brucella melitensis Isolated from Patients in Erbil, Iraq

Bushra K. Amin; Khulod I. Hassan

doi:10.14500/aro.10306

Bushra K. Amin Department of Biology, College of Science, Salahaddin University, Erbil, Kurdistan Region http://orcid.org/0000-0002-9871-3521
Khulod I. Hassan Department Food Science and Human Nutrition, College of Agriculture, University of Sulaimani, Kurdistan Region http://orcid.org/0000-0003-0114-4009

Keywords: Brucellosis, Biochemical test, Brucella melitensis, Polymerase chain reaction

Abstract

Brucellosis is a reemerging infectious zoonotic disease of worldwide importance. In the Kurdistan Region of Iraq, it is a widely spread disease and remains a challenging health problem. This disease is mainly caused by Brucella melitensis, in human. For confirmation of these isolates, a study was performed, by isolation and molecular typing of Brucella Spp. from human patients in Rizgari Hospital at Erbil city (Iraq), between March 2014 and November 2016. One hundred sixty seven samples of blood collected from patients suspected for brucellosis, one hundred twenty one samples from these were recorded as genus of Brucella, using biochemical test and confirmed by applying polymerase chain reaction (PCR), using genus specific primer for omp31 gene which was specific for B. melitensis. These results support using molecular method that based on PCR as diagnostic test for the control of brucellosis in Erbil. Further studies are needed from different geographical areas of the country with different level of endemicity to plan and execute control strategies against human brucellosis.

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Author Biographies

Bushra K. Amin, Department of Biology, College of Science, Salahaddin University, Erbil, Kurdistan Region

Bushra Qarasnji got her B.Sc. degree in Microbiology, M.Sc. degree in biotechnology and Ph.D. degree in Biotechnology (gene engineering) from Biotechnology Department, College of Science, University of Baghdad. Recently, she is an Assistant Professor at the Biology department, Salahaddin University-Erbil. Her research interests are in the Biotechnology and molecular biology fields.

Khulod I. Hassan, Department Food Science and Human Nutrition, College of Agriculture, University of Sulaimani, Kurdistan Region

Dr. Khulod is an Assistant Professor of molecular biotechnology at the department of food science and human nutrition, college of agriculture at University of Sulamani, and received her Ph.D. degree in Molecular genetics from College of Science at Baghdad University in 2002. In 2006 she joined University of Sulamani as staff member in Department of food Science and human Nutrition, College of agricultural till present. Her researches are focused on the application of polymerase chain reaction (PCR) that based on DNA extraction from deferent organisms for deferent purpose including detection and rapid identification of any species. She participated in many international conferences, and published twenty papers in high ranking International Journals.

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