Application of Random Amplified Polymorphic DNA Markers for Genetic Diversity Assessment of Pomegranate (Punica granatum L.) Cultivars in Duhok Governorate – Kurdistan of Iraq

  • Dalal Y. Sinjare Department of Scientific Research Center, College of Science, University of Duhok, Kurdistan Region
  • Sabrya J. Zubair Department of Scientific Research Center, College of Science, University of Duhok, Kurdistan Region
  • Avesta M. Ali Department of Scientific Research Center, College of Science, University of Duhok, Kurdistan Region
  • Jaladet M.S. Jubrael Department of Scientific Research Center, College of Science, University of Duhok, Kurdistan Region
DOI: https://doi.org/10.14500/aro.10078
Keywords: Genetic diversity, random amplified polymorphic DNA markers, pomegranate

Abstract

Random amplified polymorphic DNA (RAPD) markers were used to investigate the genetic diversity among 13 cultivars of pomegranate cultivated in Duhok governorate, Kurdistan region/Iraq. Unweighed pairgroup method average clustering divided the 13 cultivars into three main groups. In RAPD analysis, 12 out of 30 employed random primers showed good amplification and polymorphism on pomegranate samples with a total of 107 bands. The percentage of polymorphic DNA bands ranged from 66.6% (OPX-17) to 100% (OPO-07, OPS-17, and OPE-18, OPP-02, OPK-08, OPL-17, and OPR-01) with an average of polymorphic rate of 94.39. According to matrix coefficient, the lowest genetic similarity was observed between Soormiz and Rash Dendikheer (0.1065). The Dereger and Kapepan populations were most similar ones with coefficient of 0.9374.

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Author Biography

Jaladet M.S. Jubrael, Department of Scientific Research Center, College of Science, University of Duhok, Kurdistan Region

Prof. Jubrael got the Higher Diploma in Genetics from the Institute of Dr. P. Groza, Cluj- Napoca/ Romania/ Academic in 1978,  the M. Sc., in Molecular Genetics from Victoria University of Manchester/ UK/ Academic in 1983, and the Ph.D. degree in Genetic Engineering from Victoria University of Manchester, UK, in 1987. Recently he is a Professor in the Department of Scientific Research Center, College of Science, University of Duhok, Kurdistan Region, Iraq.

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ARO Journal: Volume 4, No. 2 (2016)
Published
2016-12-23
How to Cite
Sinjare, D. Y., Zubair, S. J., Ali, A. M. and Jubrael, J. M. (2016) “Application of Random Amplified Polymorphic DNA Markers for Genetic Diversity Assessment of Pomegranate (Punica granatum L.) Cultivars in Duhok Governorate – Kurdistan of Iraq”, ARO-THE SCIENTIFIC JOURNAL OF KOYA UNIVERSITY, 4(2), pp. 43-46. doi: 10.14500/aro.10078.
Issue
Vol. 4 No. 2 (2016): Issue Seven
Section
Articles

Copyright (c) 2016 Dalal Y. Sinjare, Sabrya J. Zubair, Avesta M. Ali, Jaladet M.S. Jubrael

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