In Silico Domain Structural Model Analysis of Coronavirus ORF1ab Polyprotein

Keywords: Coronavirus, Ligand, Modeling, ORF1ab


The world today is battling with a coronavirus infection that is considered a global pandemic. Coronavirus infection is mainly attribute to the varying technique of the replication and release of different genomic components of the virus. The present study aims to establish the physical and chemical features, as well as the basic structural and functional properties of Coronavirus ORF1ab domain. A molecular approach was adopt in this study using the Swiss Model and Phyre2 server whereas the prediction of the active ligand binding sites was done using Phyre2. The analysis of the structure of the protein showed that it has good structural and heat stability, as well as better hydrophilic features and acidic in nature. Based on the Homology modeling, only two binding active sites were noted with catalytic function being mediated by Zn2+ as the metallic heterogeneous ligand for binding sites prediction. The proteins mostly exhibited helical secondary configurations. This study can help in predicting and understanding the role of this domain protein in active coronavirus infection.


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Author Biographies

Mohammed I. Jameel, Department of Medical Microbiology, Faculty of Science and Health, Koya University, Koya KOY45, Kurdistan Region – F.R. Iraq

Mohammed I. Jameel is a Lecturer at the Department of Medical Microbiology, Faculty of Science and Health at Koya University, Kurdistan Region, F.R. Iraq. He got a B.Sc. degree in Biotechnology and the M.Sc. degree in Bioinformatics. His research interest includes microarray, whole genome sequencing data analysis, Metagenomics and R programming.

Rabar J. Noori, Department of Medical Microbiology, Faculty of Science and Health, Koya University, Koya KOY45, Kurdistan Region – F.R. Iraq

Rabar J. Noori is a Lecturer in the Department of Medical Microbiology, Faculty of Science and Health at Koya University, Kurdistan Region, F.R. Iraq. He got the B.Sc. degree in Agriculture and the M.Sc. degree in Agriculture for sustainable development. His research interest includes: water and soil microbiology, Integrated pest management. Mr. Rabar is a member of Kurdistan Agricultural Engineering Union.

Soma F. Rasul, Department of Medical Microbiology, Faculty of Science and Health, Koya University, Koya KOY45, Kurdistan Region – F.R. Iraq
Soma Fatah Rasul is a Lecturer at the Department of Medical microbiology, Faculty of Science and Health, Koya University. She got the B.Sc. degree in agriculture machinery from Koya University, the M.Sc. degree in agriculture engineering from USQ-Australia. Her research interests are in agriculture science, food science, post harvesting, precision agriculture and smart farming and renewable energy.  


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How to Cite
Jameel, M. I., Noori, R. J. and Rasul, S. F. (2022) “In Silico Domain Structural Model Analysis of Coronavirus ORF1ab Polyprotein”, ARO-THE SCIENTIFIC JOURNAL OF KOYA UNIVERSITY, 10(2), pp. 7-10. doi: 10.14500/aro.10829.