In Vitro Screening of Antibacterial Properties of Rhus coriaria and Origanum vulgare Against Some Pathogenic Bacteria

Hêro F.S Akrayi; Rebwar M.H. Salih; Pishtiwan A. Hamad

doi:10.14500/aro.10085

Hêro F.S Akrayi Department of Biology, College of Education, Salahaddin University, Kurdistan Region.
Rebwar M.H. Salih Department of Biology, College of Education, Salahaddin University, Kurdistan Region.
Pishtiwan A. Hamad Department of Biology, College of Education, Salahaddin University, Kurdistan Region.

Keywords: Antibacterial agents, antimitotic index, E. coli, K. pneumoniae, plant extracts, P. aeruginosa, P. mirabilis.

Abstract

This study investigates the antibacterial property of Rhus coriaria (Sumac) and Origanum vulgare (Jatra) aqueous extracts against Escherichia coli ATCC 25922, Klebsiella pneumoniae, Proteus mirabilis and Pseudomonas aeruginosa ATCC 27835. Results confirm the resistance of the bacterial isolates against more than three antibiotics. The aqueous extract of R. coriaria showed the highest activity as an inhibitor against tested bacteria, while the aqueous extract of O. vulgare shows low effect against the above mentioned bacteria. MIC for R. coriaria and O. vulgare aqueous extracts were determined for four bacterial isolates. The MIC of O. vulgare against tested bacteria was >12%, while the MIC of R. coriaria was 4% for E. coli, <0.025% for both K. pneumoniae and P. mirabilis and 2% for P. aeruginosa. The phytochemical groups of both plants extract were analyzed; the results indicated both plants contain tannins, phenols and saponins, flavonoid, alkaloid, and phlobatanin. The antimitotic effect of both plants extracts were investigated on Allium cepa, and the extracts showed inhibitory role in the root growth in contrast to the control when grown in the tap water for 5 days. In addition, the 24 hours treatment of grown roots in tap water with both extracts resulted in significant decrease in mitotic index.

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Author Biographies

Hêro F.S Akrayi, Department of Biology, College of Education, Salahaddin University, Kurdistan Region.

Hêro F.S Akrayi is working at Department of Biology, College of Education, Salahaddin University–Erbil. She has a B.Sc. in Biology and M.Sc. degree in Microbiology from Salahaddin University–Erbil in 2002 and 2007, respectively. From 2004 to 2005 she worked as a Biologist. Since 2007 she has worked as a Lecturer in the Department of Biology, College of Education, Salahaddin University – Erbil, Iraq.

Rebwar M.H. Salih, Department of Biology, College of Education, Salahaddin University, Kurdistan Region.

Rebwar M.H. Salih received B.Sc. Degree and M.S. Degrees in Biology and Microbiology (Bacteriology) from Salahaddin University–Erbil in 2003 and 2008, respectively. From 2003 to 2006 he worked as Biologist. Since 2010 he has worked as an Assistant Lecturer in the Department of Biology, College of Education, Salahaddin University–Erbil, Iraq.

Pishtiwan A. Hamad, Department of Biology, College of Education, Salahaddin University, Kurdistan Region.

Pishtiwan A. Hamad is a lecturer at Department of Biology, College of Education, Salahaddin University–Erbil. He has a B.Sc. in Biology and M.Sc. degree in Microbiology from Salahaddin University–Erbil in 2005 and 2010, respectively. From 2006 to 2008 he worked as Biologist. Since 2011 he has worked as an Assistant Lecturer in the Department of Biology, College of Education, Salahaddin University – Erbil, Iraq.

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