Glucuronidase Gene

A Strong Evidence of a Novel Interaction of Glucuronidase-labeled Gluconacetobacter diazotrophicus with Spinach, Spinacia oleracea L. Seedlings

Keywords: Glucuronidase protocol, Gluconacetobacter diazotrophicus, Interaction, Nitrogen fixation, Spinach


Gluconacetobacter diazotrophicus lives inside plant tissue cells in the form of colonies and excretes about half of the fixed nitrogen, which offers potential power that improves plant growth. The aim of this study is to find the interaction of glucuronidase (GUS)-labeled G. diazotrophicus with spinach seedlings and the detection of GUS genes using X-gluc dye (5-bromo-4-chloro-3-indolyl-β-D- glucuronic acid). The GUS protocol is used to detect GUS-labeled G. diazotrophicus in spinach seedling tissues by chemical detection using X-gluc dye. The results show that the spinach seedlings are successfully infected with GUS-labeled G. diazotrophicus , with the survival of the seedlings throughout their growth period and an improvement in the growth of pollinated seedlings. The outcomes of the microscopic inspection of the root slices reveal the presence of bacterial cells at the root tips and their concentration in the area of the cell walls of the peripheral cells. Furthermore, the findings of microscopic examinations of longitudinal sections for cotyledons show the presence of a number of bacteria within epidermal cell walls. This indicates that the determinants of the interaction between these bacteria and spinach seedlings are suitable for the expression of the gene responsible for the formation of the nitrogenase enzyme.


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Author Biographies

Hikmat M. Masyab, Department of Biology, Faculty of Science and Health, Koya University, Koya KOY45, Kurdistan Region - F.R. Iraq

Hikmat M. Masyab is an Assistant Prof at the Department of Biology, Faculty of Science and health, Koya
University. He got the B.Sc. degree in Biology, the M.Sc. degree in Biology/Botany and the Ph.D. degree in
Biotechnology. His research interests are in Biotechnology, Plant Tissue Culture and Genetic Engineering.

Qutaiba S. Al-Nema, Department of Biology, College Education for Pure Sciences, University of Mosul, Mosul, F.R Iraq

Qutaiba S. Al-Nema is a Professor at the Department of Biology, College Education for Pure Sciences, University of Mosul. He got the B.Sc. degree in Biology, the M.Sc. degree in Biology/Botany and the Ph.D. degree in Biotechnology. His research interests are in Biotechnology, Plant Tissue Culture and Genetic Engineering.

Mozahim Q. Al-Mallah, Department of MLT, Al-Hadbaa University College, Mosul, F..R. Iraq

Mozahim K. Al-Mallah is a Professor at the Department of Medical Laboratory Techniques, Alhadba University College, Mosul. He got the B.Sc. degree in Plant protection, the M.Sc. degree in Virology and the Ph.D. degree in Genetic Engineering. His research interests are in Biotechnology, Plant Tissue Culture and Genetic Engineering.


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How to Cite
Masyab, H. M., Al-Nema, Q. S. and Al-Mallah, M. Q. (2024) “Glucuronidase Gene: A Strong Evidence of a Novel Interaction of Glucuronidase-labeled Gluconacetobacter diazotrophicus with Spinach, Spinacia oleracea L. Seedlings”, ARO-THE SCIENTIFIC JOURNAL OF KOYA UNIVERSITY, 12(1), pp. 8-12. doi: 10.14500/aro.11239.
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