Role of Laser Produced Silver Nanoparticles in Reversing Antibiotic Resistance in Some MultidrugResistant Pathogenic Bacteria

Abubaker H. Hamad; Mahmoud A. Chawsheen; Ahmed A. Al-Naqshbandi

doi:10.14500/aro.10877

Abubaker H. Hamad Department of General Science, Faculty of Education, Soran University, Erbil, Kurdistan Region - F.R. Iraq https://orcid.org/0000-0003-0151-5228
Mahmoud A. Chawsheen Department of General Science, Faculty of Education, Soran University, Erbil, Kurdistan Region - F.R. Iraq https://orcid.org/0000-0003-2277-1984
Ahmed A. Al-Naqshbandi Department of Laboratory, Rizgary Teaching Hospital, Erbil, Kurdistan Region - F.R. Iraq https://orcid.org/0000-0002-0843-7958

Keywords: Ampicillin, Antibacterial activity, Laser ablation, Nanosecond laser, Silver nanoparticles

Abstract

Silver nanoparticles (Ag NPs) were produced through nanosecond laser in deionized water. These nanoparticles were characterized by UV–VIS spectrometer and transmission electron microscopy. VITEK®2 compact system was used to identify Escherichia coli (ESBL strain) and Staphylococcus aureus (MRSA strain) as multidrug-resistance (MDR) bacteria. The antibacterial activity of Ag NPs, ampicillin (AMP), and their combinations was tested against both bacterial isolates through standard microbiological culturing techniques. Our data show that both of E. coli and S. aureus were highly resistant to AMP. Ag NPs alone reduced growth in both bacterial isolates considerably. Growth declined drastically in both bacteria when AMP was used in combination with Ag NPs. The minimal inhibitory concentration of combined agents for E. coli was 20 µg/ml Ag NPs + 1 mg AMP/ml and for S. aureus was 10 µg/ml Ag NPs + 1 mg AMP/ml. The results show that the Ag NPs have great potentials in enhancing the antimicrobial activities of drugs that used to be ineffective against MDR bacteria. Administering combinations of antibiotic(s) with AgNPs may help in treating patients suffering from infections caused by MDR bacteria. Further in vivo and in vitro investigations are required to evaluate the side effects of these combinations.

Downloads

Download data is not yet available.

Author Biographies

Abubaker H. Hamad, Department of General Science, Faculty of Education, Soran University, Erbil, Kurdistan Region - F.R. Iraq

Abubaker Hassan Hamad is an Assistant Prof. at the Department of General Science, Faculty of Education, Soran University. He got the B.Sc. degree in Physics, the M.Sc. degree in Lasers and the Ph.D. degree in nanotechnology. His research interests are in nanotechnology, laser material interaction and Nanoparticles for bio-application.

Mahmoud A. Chawsheen, Department of General Science, Faculty of Education, Soran University, Erbil, Kurdistan Region - F.R. Iraq

Mahmoud A. Chawsheen is Assistant Prof. at the Department of General Sciences, Faculty of Education, Soran University. He got the B.Sc. degree in Biological Sciences, the M.Sc. degree in Cell Biology, and the Ph.D. degree Cell Biology as well. His research interests are in Cancer, Multi-drug Resistance, and Cell Signaling. Dr. Mahmoud is a member of the European Association for Cancer Research and The Royal Microscopical Society.

Ahmed A. Al-Naqshbandi, Department of Laboratory, Rizgary Teaching Hospital, Erbil, Kurdistan Region - F.R. Iraq

Ahmed A. Al-Naqshbandi, is a Microbiology Specialist at the Department of Laboratory (Microbiology Unit), Rizgary Teaching Hospital, Director of Health - Erbil. He got the B.Sc. degree in Biology, the M.Sc. degree in Microbiology (Immunology) and he is currently a Ph.D. student at Dept. of Biology, College of Education, Salahaddin University. His research interests are in Immunology, Bacteriology and Molecular Biology. Mr. Ahmed is a member of the Kurdistan Biology Syndicate.

References

Allahverdiyev, A.M., Kon, K.V., Abamor, E.S., Bagirova, M. and Rafailovich,M., 2011. Coping with antibiotic resistance: Combining nanoparticles with antibiotics and other antimicrobial agents. Expert Review of Anti-Infective Therapy, 9, pp.1035-1052.

Al-Naqshbandi, A.A., Chawsheen, M.A. and Abdulqader, H.H., 2019. Prevalence and antimicrobial susceptibility of bacterial pathogens isolated from urine specimens received in Rizgary hospital-Erbil. Journal of Infection and Public Health, 12, pp.330-336.

Al-Naqshbandi, A.A., Hassan, H.A., Chawsheen, M.A. and Qader, H.H.A., 2021. Categorization of bacterial pathogens present in infected wounds and their antibiotic resistance profile recovered from patients attending rizgary hospitalErbil. ARO The Scientific Journal of Koya University, 9, pp.64-70.

Al-Ogaidi, I.A. Z., 2017. Detecting the antibacterial activity of green synthesized silver (Ag) nanoparticles functionalized with ampicillin (Amp). Baghdad Science Journal, 14, pp.117-125.

Biggs, B.A. and Kucers, A., 1986. Penicillins and related drugs. The Medical Journal of Australia, 145, pp.607-611.

Brasil, M.S., Filgueiras, A.L., Campos, M.B., Neves, M.S., Eugênio, M., Sena, L.A., Sant’anna, C.B., Silva, V.L.D., Diniz, C.G. and Sant’ana, A.C., 2018. Synergism in the antibacterial action of ternary mixtures involving silver nanoparticles, chitosan and antibiotics. Journal of the Brazilian Chemical Society, 29, pp.2026-2033.

Brown, A.N., Smith, K., Samuels, T.A., Lu, J., Obare, S.O. and Scott, M.E., 2012. Nanoparticles functionalized with ampicillin destroy multiple-antibioticresistant isolates of Pseudomonas aeruginosa and Enterobacter aerogenes and methicillin-resistant Staphylococcus aureus. Applied. Environmental. Microbiology, 78, 2768-2774.

Chaloupka, K., Malam, Y. and Seifalian, A.M., 2010. Nanosilver as a new generation of nanoproduct in biomedical applications. Trends in Biotechnology, 28, pp.580-588.

Chawsheen, M.A., AL-Naqshbandi, A.A. and Abdulqader, H.H., 2020. Bacterial profile and antimicrobial susceptibility of isolates recovered from lower respiratory tract infection for patients in Rizgary hospital, Erbil. Aro-the Scientific Journal of Koya University, 8, pp.64-70.

Dakal, T.C., Kumar, A., Majumdar, R.S. and Yadav, V., 2016. Mechanistic basis of antimicrobial actions of silver nanoparticles. Frontiers in Microbiology, 7, pp.1831.

Fayaz, A.M., Balaji, K., Girilal, M., Yadav, R., Kalaichelvan, P.T. and Venketesan, R., 2010. Biogenic synthesis of silver nanoparticles and their synergistic effect with antibiotics: A study against gram-positive and gramnegative bacteria. Nanomedicine, 6, pp.103-109.

Feng, Q.L., Wu, J., Chen, G.Q., Cui, F.Z., Kim, T.N. and Kim, J.O., 2000. A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus. Journal of Biomedical Materials Research, 52, pp.662-668.

Greenberg, M., Kuo, D., Jankowsky, E., Long, L., Hager, C., Bandi, K., MA, D., Manoharan, D., Shoham, Y. and Harte, W., 2018. Small-molecule AgrA inhibitors F12 and F19 act as antivirulence agents against gram-positive pathogens. Scientific Reports, 8, pp.1-12.

Haider, A. and Kang, I.K., 2015. Preparation of silver nanoparticles and their industrial and biomedical applications: A comprehensive review. Advances in Materials Science and Engineering, 2015, p.165257.

Hajipour, M.J., Fromm, K.M., Ashkarran, A.A., De Aberasturi, D.J., De Larramendi, I.R., Rojo, T., Serpooshan, V., Parak, W.J. and Mahmoudi, M., 2012. Antibacterial properties of nanoparticles. Trends in Biotechnology, 30, pp.499-511.

Hamad, A., Li, L. and Liu, Z., 2015a. A comparison of the characteristics of nanosecond, picosecond and femtosecond lasers generated Ag, TiO2 and Au nanoparticles in deionised water. Applied Physics A, 120, pp.1247-1260.

Hamad, A., Li, L., Liu, Z., Zhong, X.L. and Wang, T., 2015c. Picosecond laser generation of Ag-TiO2 nanoparticles with reduced energy gap by ablation in ice water and their antibacterial activities. Applied Physics A, 119, pp.1387-1396.

Hamad, A., Li, l., Liu, Z., Zhong, X.L., Liu, H. and Wang, T., 2015b. Generation of silver titania nanoparticles from an Ag-Ti alloy via picosecond laser ablation and their antibacterial activities. Royal Society of Chemistry Advances, 5, pp.72981-72994.

Hamad, A.H, Khashan, K.S. and Hadi, A.A., 2020. Silver nanoparticles and silver ions as potential antibacterial agents. Journal of Inorganic and Organometallic Polymers and Materials, 30, 1-18.

Holt, K.B. and Bard, A.J., 2005. Interaction of silver (I) ions with the respiratory chain of Escherichia coli: An electrochemical and scanning electrochemical microscopy study of the antimicrobial mechanism of micromolar Ag+. Biochemistry, 44, pp.13214-13223.

Hwang, I.S., Hwang, J.H., Choi, H., Kim, K.J. and Lee, D.G., 2012. Synergistic effects between silver nanoparticles and antibiotics and the mechanisms involved. Journal of Medical Microbiology, 61, pp.1719-1726.

Juan, L., Zhimin, Z., Anchun, M., Lei, L. and Jingchao, Z., 2010. Deposition of silver nanoparticles on titanium surface for antibacterial effect. International Journal of Nanomedicine, 5, p.261.

Katzung, B.G. and Trevor, A.J., 2012. Basic and Clinical Pharmacology. McGraw Hill Professional, New York.

Krzyżaniak, N., Pawłowska, I. and Bajorek, B., 2016. Review of drug utilization patterns in NICU s worldwide. Journal of Clinical Pharmacy and Therapeutics, 41, pp.612-620.

Liao, C., Li, Y. and Tjong, S.C., 2019. Bactericidal and cytotoxic properties of silver nanoparticles. International Journal of Molecular Sciences, 20, 449.

Mafuné, F., Kohno, J.Y., Takeda, Y., Kondow, T. and Sawabe, H., 2000. Structure and stability of silver nanoparticles in aqueous solution produced by laser ablation. The Journal of Physical Chemistry B, 104, pp.8333-8337.

Marassi, V., Di Cristo, L., Smith, S.G.J., Ortelli, S., Blosi, M., Costa, A.L., Reschiglian, P., Volkov, Y. and Prina-Mello, A., 2018. Silver nanoparticles as a medical device in healthcare settings: A five-step approach for candidate screening of coating agents. Royal Society Open Science, 5, pp.171113.

Morones, J.R., Elechiguerra, J.L., Camacho, A., Holt, K., Kouri, J.B., Ramírez, J.T. and Yacaman, M.J., 2005. The bactericidal effect of silver nanoparticles. Nanotechnology, 16, pp.2346-2353.

Opal, S.M., 2016. Non-antibiotic treatments for bacterial diseases in an era of progressive antibiotic resistance. Critical Care, 20, p.397.

Pandey, J.K., Swarnkar, R., Soumya, K.K., Dwivedi, P., Singh, M.K., Sundaram, S. and Gopal, R., 2014. Silver nanoparticles synthesized by pulsed laser ablation: As a potent antibacterial agent for human enteropathogenic gram-positive and gram-negative bacterial strains. Applied Biochemistry and Biotechnology, 174, pp.1021-1031.

Perito, B., Giorgetti, E., Marsili, P. and Muniz-Miranda, M., 2016. Antibacterial activity of silver nanoparticles obtained by pulsed laser ablation in pure water and in chloride solution. Beilstein Journal of Nanotechnology, 7, pp.465-473.

Poh, T.Y., Ali, N.A.B., Mac Aogáin, M., Kathawala, M.H., Setyawati, M.I., Ng, K.W. and Chotirmall, S.H., 2018. Inhaled nanomaterials and the respiratory microbiome: Clinical, immunological and toxicological perspectives. Particle and Fibre Toxicology, 15, p.46.

Prasher, P., Singh, M. and Mudila, H., 2018. Silver nanoparticles as antimicrobial therapeutics: Current perspectives and future challenges. 3 Biotechnology, 8, p.411.

Rai, M., Yadav, A. and Gade, A., 2009. Silver nanoparticles as a new generation of antimicrobials. Biotechnology Advances, 27, pp.76-83.

Rajesh, S., Dharanishanthi, V. and Kanna, A.V., 2015. Antibacterial mechanism of biogenic silver nanoparticles of Lactobacillus acidophilus. Journal of Experimental Nanoscience, 10, pp.1143-1152.

Russell, A. and Hugo, W., 1994. Antimicrobial activity and action of silver. Progress in Medicinal Chemistry, 31, pp.351-70.

Shrivas, K., Sahu, J., Maji, P. and Sinha, D., 2017. Label-free selective detection of ampicillin drug in human urine samples using silver nanoparticles as a colorimetric sensing probe. New Journal of Chemistry, 41, pp.6685-6692.

Shrivastava, S., Bera, T., Roy, A., Singh, G., Ramachandrarao, P. and Dash, D., 2007. Characterization of enhanced antibacterial effects of novel silver nanoparticles. Nanotechnology, 18, p.225103.

Simbine, E.O., Rodrigues, L.D.C., Lapa-Guimaraes, J., Kamimura, E.S., Corassin, C.H. and De Oliveira, C.A.F., 2019. Application of silver nanoparticles in food packages: A review. Food Science and Technology, 67, pp.1942-1956.

Sportelli, M.C., Izzi, M., Volpe, A., Clemente, M., Picca, R.A., Ancona, A., Lugarà, P.M., Palazzo, G. and Cioffi, N., 2018. The pros and cons of the use of laser ablation synthesis for the production of silver nano-antimicrobials. Antibiotics (Basel), 7, p.67.

Weber-Dąbrowska, B., Zimecki, M., Kruzel, M., Kochanowska, I. and ŁusiakSzelachowska, M., 2006. Alternative therapies in antibiotic-resistant infection. Advances Medical Sciences, 51, pp.242-244.

World Health Organization, 2020. Antimicrobial Resistance. Geneva: World Health Organization. Available from: https://www.who.int/news-room/factsheets/detail/antimicrobial-resistance [Last accessed on 2020 Jul 22].

Zamora, L.L. and Perez-Gracia, M.T., 2012. Using digital photography to implement the McFarland method. Journal of the Royal Society Interface, 9, pp.1892-1897.

Zhang, X.F., Liu, Z.G., Shen, W. and Gurunathan, S., 2016. Silver nanoparticles: Synthesis, characterization, properties, applications, and therapeutic approaches. International Journal of Molecular Sciences, 17, p.1534.