Role of Laser Produced Silver Nanoparticles in Reversing Antibiotic Resistance in Some MultidrugResistant Pathogenic Bacteria

Keywords: Ampicillin, Antibacterial activity, Laser ablation, Nanosecond laser, Silver nanoparticles


Silver nanoparticles (Ag NPs) were produced through nanosecond laser in deionized water. These nanoparticles were characterized by UV–VIS spectrometer and transmission electron microscopy. VITEK®2 compact system was used to identify Escherichia coli (ESBL strain) and Staphylococcus aureus (MRSA strain) as multidrug-resistance (MDR) bacteria. The antibacterial activity of Ag NPs, ampicillin (AMP), and their combinations was tested against both bacterial isolates through standard microbiological culturing techniques. Our data show that both of E. coli and S. aureus were highly resistant to AMP. Ag NPs alone reduced growth in both bacterial isolates considerably. Growth declined drastically in both bacteria when AMP was used in combination with Ag NPs. The minimal inhibitory concentration of combined agents for E. coli was 20 µg/ml Ag NPs + 1 mg AMP/ml and for S. aureus was 10 µg/ml Ag NPs + 1 mg AMP/ml. The results show that the Ag NPs have great potentials in enhancing the antimicrobial activities of drugs that used to be ineffective against MDR bacteria. Administering combinations of antibiotic(s) with AgNPs may help in treating patients suffering from infections caused by MDR bacteria. Further in vivo and in vitro investigations are required to evaluate the side effects of these combinations.


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Author Biographies

Abubaker H. Hamad, Department of General Science, Faculty of Education, Soran University, Erbil, Kurdistan Region - F.R. Iraq

Abubaker Hassan Hamad is an Assistant Prof. at the Department of General Science, Faculty of Education, Soran University. He got the B.Sc. degree in Physics, the M.Sc. degree in Lasers and the Ph.D. degree in nanotechnology. His research interests are in nanotechnology, laser material interaction and Nanoparticles for bio-application.

Mahmoud A. Chawsheen, Department of General Science, Faculty of Education, Soran University, Erbil, Kurdistan Region - F.R. Iraq

Mahmoud A. Chawsheen is Assistant Prof. at the Department of General Sciences, Faculty of Education, Soran University. He got the B.Sc. degree in Biological Sciences, the M.Sc. degree in Cell Biology, and the Ph.D. degree Cell Biology as well. His research interests are in Cancer, Multi-drug Resistance, and Cell Signaling. Dr. Mahmoud is a member of the European Association for Cancer Research and The Royal Microscopical Society.

Ahmed A. Al-Naqshbandi, Department of Laboratory, Rizgary Teaching Hospital, Erbil, Kurdistan Region - F.R. Iraq

Ahmed A. Al-Naqshbandi, is a Microbiology Specialist at the Department of Laboratory (Microbiology Unit), Rizgary Teaching Hospital, Director of Health - Erbil. He got the B.Sc. degree in Biology, the M.Sc. degree in Microbiology (Immunology) and he is currently a Ph.D. student at Dept. of Biology, College of Education, Salahaddin University. His research interests are in Immunology, Bacteriology and Molecular Biology. Mr. Ahmed is a member of the Kurdistan Biology Syndicate.


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How to Cite
Hamad, A. H., Chawsheen, M. A. and Al-Naqshbandi, A. A. (2022) “Role of Laser Produced Silver Nanoparticles in Reversing Antibiotic Resistance in Some MultidrugResistant Pathogenic Bacteria”, ARO-THE SCIENTIFIC JOURNAL OF KOYA UNIVERSITY, 10(1), pp. 104-110. doi: 10.14500/aro.10877.