Extraction and Determination of Oxymatrine Pesticide in Environmental Sample and in its Formulation using High-Performance Liquid Chromatography

Ihsan M. Shaheed; Saadiyah A. Dhahir

doi:10.14500/aro.10666

Ihsan M. Shaheed Department of Chemistry, Faculty of Science, University of Baghdad,Baghdad, Iraq http://orcid.org/0000-0002-8154-3407
Saadiyah A. Dhahir Department of Chemistry, Faculty of Science for Women, University of Baghdad, Baghdad, Iraq http://orcid.org/0000-0001-9674-6613

Keywords: Dispersive, Oxymatrine, Reverse-phase high-performance liquid chromatography, UV visible

Abstract

The quinolizindine alkaloid compound, oxymatrine pesticide, was analysis in the river water samples collected from different agriculture areas in the Iraqi city of Kerbala and also in its formulation using developed reverse-phase high-performance liquid chromatography method. Acetonitrile:methanol (60:40 v/v) was chosen as mobile phase at pH (7.0), flow rate 0.5 mL/min, and 20 µL as volume injection. Modified ecological-friendly method, dispersive liquid-liquid microextraction, was used for the extraction of oxymatrine from water samples. Linearity study was constructed from 0.1 to 70 μg/mL at λmax 205 nm. The limit of detection and limit of quantification were 0.025 and 0.082 μg/mL, respectively, and the relative standard deviation (RSD) % was 0.518%. Three spiked levels of concentration (20.0, 40.0, and 70.0 μg/mL) were used for the validation method. The percentage recovery for the three spiked samples was ranged between 98.743 and 99.432 and the RSD% was between 0.051 and 0.202%, the formulation studies of oxymatrine between 99.487 and 99.798, and the RSD% was ranged from 0.045 to 0.057%. The developed method can be used accurately and selectively for the determination of oxymatrine in environmental samples and in the formulation.

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Author Biographies

Ihsan M. Shaheed, Department of Chemistry, Faculty of Science, University of Baghdad,Baghdad, Iraq

Ihsan Mahdi Shaheed is a Lecturer at the Department of Chemistry, Faculty/College of Science, Kerbala University. He got the B.Sc. degree in 1994, the M.Sc. degree in 2013 and the Ph.D. degree in 2020. His research interests are in analytical chemistry, environmental and pharmaceutical chemistry.

Saadiyah A. Dhahir, Department of Chemistry, Faculty of Science for Women, University of Baghdad, Baghdad, Iraq

Saadiyah Ahmed Dhahir is a Prof. at the Department of Chemistry College of science for women, Baghdad University. She got the B.Sc. degree in chemistry, the M.Sc. degree in Biochemistry and the Ph.D. degree in Analytical chemistry. Her research interests are in Environmental chemistry analysis, Pharmaceutical chemistry analysis and Photo catalyst chemistry analysis of organic polluted. Dr. Saadiyah is a member of Baghdad science journal.

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