Analyzing Colorectal Cancer at the Molecular Level through Next-generation Sequencing in Erbil City

Vyan A. Qadir; Kamaran K. Abdoulrahman

doi:10.14500/aro.11495

Vyan A. Qadir Department of Chemistry, Faculty of Science and Health, Koya University, Koya KOY45, Kurdistan Region - F.R. Iraq https://orcid.org/0000-0003-1346-1722
Kamaran K. Abdoulrahman Department of Chemistry, College of Science, Salahaddin University-Erbil, Kurdistan Region, Iraq http://orcid.org/0000-0002-5982-2928

Keywords: Colorectal, Cancer, Gene, Molecular level, Mutation

Abstract

Colorectal cancer (CRC) ranks as the third leading cause of cancer-related deaths globally. It is characterized as a genomic disorder marked by diverse genomic anomalies, including point mutations, genomic rearrangements, gene fusions, and alterations in chromosomal copy numbers. This research aims to identify previously undisclosed genetic variants associated with an increased risk of CRC by employing next-generation sequencing technology. Genomic DNA was extracted from blood specimens of five CRC patients. The sequencing data of the samples are utilized for variant identification. In addition, the Integrative Genomic Viewer software (IGV) is used to visualize the identified variants. Furthermore, various in silico tools, including Mutation Taster and Align GVGD, are used to predict the potential impact of mutations on structural features and protein function. Based on the findings of this research, 12 different genetic variations are detected among individuals with CRC. Inherited variations are located within the following genes: MSH6, MSH2, PTPRJ, PMS2, TP53, BRAF, APC, and PIK3CA.

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Author Biographies

Vyan A. Qadir, Department of Chemistry, Faculty of Science and Health, Koya University, Koya KOY45, Kurdistan Region - F.R. Iraq

Vyan A. Qadir is a Lecturer at the Department of Medicine, Faculty of General Medicine, Koya University. She got the B.Sc. degree in Chemistry, the M.Sc. degree in Clinical Biochemistry. Her research interests are in Biochemistry, Clinical Biochemistry and Medical Microbiology.

Kamaran K. Abdoulrahman, Department of Chemistry, College of Science, Salahaddin University-Erbil, Kurdistan Region, Iraq

Kamaran K. Abdoulrahman is an Assistant Professor at the Department of Chemistry, College of Science, Salahaddin University. He got the B.Sc. degree in Chemistry, the M.Sc. degree in Biochemistry and the Ph.D. degree in Biochemistry. His research interests are in Biochemistry, Clinical Biochemistry and Molecular Biology.

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