Oxidative Stress Assessment in Colorectal Cancer Patients
Erbil Population Study
Abstract
Colorectal cancer (CRC), a global health challenge, exhibits rising incidence in low-income nations due to lifestyle changes. Oxidative stress, indicated by reactive oxygen species imbalance and Malondialdehyde (MDA), is linked to CRC. This study investigates oxidative stress markers, antioxidant enzymes, genetic markers, cellular regulation markers, and Vitamin E in CRC patients in Erbil. Ninety CRC patients and 30 healthy controls provided blood samples, processed and stored at –20°C. Enzyme-linked immunosorbent assay kits quantified oxidative stress, antioxidant markers, and Vitamin E. Oxidative stress markers showed significant differences, with elevated MDA and 8-hydroxy-2’-deoxyguanosine levels in patients. Nitrotyrosine exhibited lower expression in patients. Antioxidant enzymes glutathione peroxidase and superoxide dismutase were enhanced in patients, while glutathione (GSH), glutathione reductase and catalase levels were significantly lower in patients. The genetic marker KRAS showed a substantial decrease in patients (<0.0001) but both adenomatous polyposis coli (APC) and CRC antigen (CCA) were higher. Serum vitamin E levels were significantly lower in patients (71.78 ± 6.368) compared to controls (142.3 ± 4.828, p < 0.0001). Elevated oxidative stress, altered enzymatic activity, significantly lower expression of KRAS, and higher expression of APC and CCA in the patient group. Furthermore, reduced Vitamin E levels were observed in the patient group, highlighting potential challenges in antioxidant defense.Downloads
References
Acevedo-León, D., Monzó-Beltrán, L., Gómez-Abril, S.Á., Estañ-Capell, N., Camarasa-Lillo, N., Perez-Ebri, M.L., Escandón-Álvarez, J., Alonso-Iglesias, E., Santaolaria-Ayora, M.L., Carbonell-Moncho, A., Ventura-Gayete, J., Pla, L., Martínez-Bisbal, M.C., Martínez-Máñez, R., Bagán-Debón, L., Viña-Almunia, A., Martínez-Santamaría, M.A., Ruiz-Luque, M., Alonso-Fernández, J., Bañuls, C., and Sáez, G., 2021. The effectiveness of glutathione redox status as a possible tumor marker in colorectal cancer. International Journal of Molecular Sciences, 22, p.6183. DOI: https://doi.org/10.3390/ijms22126183
Acevedo-León, D., Monzó-Beltrán, L., Perez-Sánchez, L., Naranjo-Morillo, E., Gómez-Abril, S.Á., Estañ-Capell, N., Bañuls, C., and Sáez, G., 2022. Oxidative stress and DNA damage markers in colorectal Cancer. International Journal of Molecular Sciences, 23, p.11664. DOI: https://doi.org/10.3390/ijms231911664
Aghabozorgi, A.S., Bahreyni, A., Soleimani, A., Bahrami, A., Khazaei, M., Ferns, G.A., Avan, A., and Hassanian, S.M.J.B., 2019. Role of adenomatous polyposis coli (APC) gene mutations in the pathogenesis of colorectal cancer; current status and perspectives. Biochimie, 157, pp.64-71. DOI: https://doi.org/10.1016/j.biochi.2018.11.003
Al-Ansari, R.F., Al-Gebori, A.M., and Sulaiman, G.M., 2020. Serum levels of zinc, copper, selenium and glutathione peroxidase in the different groups of colorectal cancer patients. Caspian Journal of Internal Medicine, 11, p.384.
Alrubaie, A., Alkhalidi, N., and Abd-Alhusain, S., 2019. A clinical study of newly-diagnosed colorectal cancer over 2 years in a gastroenterology center in Iraq. Journal of Coloproctology, 39, pp.217-222. DOI: https://doi.org/10.1016/j.jcol.2019.05.010
Altoriah, K.M.J., Jumaah, A., Abdulhussein, A.A., Aljanabi, A.A.H., Al-Haddad, H.S., Al-Quzweni, A., and Hadi, N., 2020. Immunohistochemical study of adenomatous polyposis coli protein in colorectal carcinoma and its precursor lesions in Iraq. Systematic Reviews in Pharmacy, 11, pp.75-79. DOI: https://doi.org/10.31838/srp.2020.5.13
Alves Ribeiro, R.R., Rolim De Brito, I., Andrade Souza, K., De Castro Souza, L., Almeida De Oliveira, T., and Weller, M., 2022. Risk of colorectal cancer in a brazilian population is differentially associated with the intake of processed meat and vitamin E. Nutrition and Cancer, 74, pp.820-829. DOI: https://doi.org/10.1080/01635581.2021.1926519
Arnold, M., Sierra, M.S., Laversanne, M., Soerjomataram, I., Jemal, A., and Bray, F., 2017. Global patterns and trends in colorectal cancer incidence and mortality. Gut, 66, pp.683-691. DOI: https://doi.org/10.1136/gutjnl-2015-310912
Arrington, A.K., Heinrich, E.L., Lee, W., Duldulao, M., Patel, S., Sanchez, J., Garcia-Aguilar, J., and Kim, J., 2012. Prognostic and predictive roles of KRAS mutation in colorectal cancer. International Journal of Molecular Sciences, 13, pp.12153-12168. DOI: https://doi.org/10.3390/ijms131012153
Balaky, H.M., 2023. Potential role of 8-hydroxyguanosine and some pro inflammatory cytokines as biomarkers in colorectal cancer Iraqi patients. Baghdad Science Journal, 20, p.0082. DOI: https://doi.org/10.21123/bsj.2022.6778
Bandookwala, M., and Sengupta, P., 2020. 3-Nitrotyrosine: A versatile oxidative stress biomarker for major neurodegenerative diseases. International Journal of Neuroscience, 130, pp.1047-1062. DOI: https://doi.org/10.1080/00207454.2020.1713776
Bardelčíková, A., Šoltys, J., and Mojžiš, J., 2023. Oxidative stress, inflammation and colorectal cancer: An overview. Antioxidants (Basel), 12, p.901. DOI: https://doi.org/10.3390/antiox12040901
Bartesaghi, S., and Radi, R., 2018. Fundamentals on the biochemistry of peroxynitrite and protein tyrosine nitration. Redox Biology, 14, pp.618-625. DOI: https://doi.org/10.1016/j.redox.2017.09.009
Basak, D., Uddin, M.N., and Hancock, J., 2020. The role of oxidative stress and its counteractive utility in colorectal cancer (CRC). Cancers (Basel), 12, p.3336. DOI: https://doi.org/10.3390/cancers12113336
Beniwal, S.S., Lamo, P., Kaushik, A., Lorenzo-Villegas, D.L., Liu, Y., and Mohanasundaram, A., 2023. Current status and emerging trends in colorectal cancer screening and diagnostics. Biosensors (Basel), 13, p.926. DOI: https://doi.org/10.3390/bios13100926
Borkowska, A., Olszewska, A., Skarzynska, W., Marciniak, M., Skrzeszewski, M., Kieda, C., and Was, H., 2022. High hemin concentration induces escape from senescence of normoxic and hypoxic colon cancer cells. Cancers (Basel), 14, p.4793. DOI: https://doi.org/10.3390/cancers14194793
Borrego, S., Vazquez, A., Dasí, F., Cerdá, C., Iradi, A., Tormos, C., Sánchez, J.M., Bagán, L., Boix, J., Camps, J., Sáez, G., and Zaragoza, C., 2013. Oxidative stress and DNA damage in human gastric carcinoma: 8-Oxo-7’8-dihydro-2’-deoxyguanosine (8-oxo-dG) as a possible tumor marker. International Journal of Molecular Sciences, 14, pp.3467-3486. DOI: https://doi.org/10.3390/ijms14023467
Bratovcic, A.J.A.S., 2020. Antioxidant enzymes and their role in preventing cell damage. Acta Scientific Nutritional Health, 4, pp.1-7.Fig. 11. Serum Vitamin E concentration level. DOI: https://doi.org/10.31080/ASNH.2020.04.0659
Brzozowa-Zasada, M., Ianaro, A., Piecuch, A., Michalski, M., Matysiak, N., and Stęplewska, K., 2023. Immunohistochemical expression of glutathione peroxidase-2 (Gpx-2) and its clinical relevance in colon adenocarcinoma patients. International Journal of Molecular Sciences, 24, p.14650. DOI: https://doi.org/10.3390/ijms241914650
Cecerska-Heryć, E., Surowska, O., Heryć, R., Serwin, N., Napiontek-Balińska, S., and Dołęgowska, B., 2021. Are antioxidant enzymes essential markers in the diagnosis and monitoring of cancer patients-a review. Clinical Biochemistry, 93, pp.1-8. DOI: https://doi.org/10.1016/j.clinbiochem.2021.03.008
Chang, D., Wang, F., Zhao, Y.S., and Pan, H.Z., 2008. Evaluation of oxidative stress in colorectal cancer patients. Biomedical and Environmental Sciences, 21, pp.286-289. DOI: https://doi.org/10.1016/S0895-3988(08)60043-4
Chatterjee, N., and Walker, G.C., 2017. Mechanisms of DNA damage, repair, and mutagenesis. Environmental and Molecular Mutagenesis, 58, pp.235-263. DOI: https://doi.org/10.1002/em.22087
Daly, C.S., 2013. The Roles of the Apc Proteins in Homeostasis and Tumourigenesis. Cardiff University, Cardiff.Demasi, M., Augusto, O., Bechara, E.J., Bicev, R.N., Cerqueira, F.M., Da Cunha, F.M., Denicola, A., Gomes, F., Miyamoto, S., Netto, L.E.S., Randall, L.M., Stevani, C.V., and Thomson, L., 2021. Oxidative modification of proteins: From damage to catalysis, signaling, and beyond. Antioxidants and Redox Signaling, 35, pp.1016-1080. DOI: https://doi.org/10.1089/ars.2020.8176
Demirci-Cekic, S., Özkan, G., Avan, A.N., Uzunboy, S., Çapanoğlu, E., and Apak, R.J., 2022. Biomarkers of oxidative stress and antioxidant defense. Journal of Pharmaceutical and Biomedical Analysis, 209, p.114477. DOI: https://doi.org/10.1016/j.jpba.2021.114477
Fedacko, J., Takahashi, T., Singh, R.B., Pella, D., Chibisov, S., Hristova, K., Pella, D., Elkilany, G.N., Tomar, R.S., and Juneja, L.R., 2019. Globalization of diets and risk of noncommunicable diseases. In: The Role of Functional Food Security in Global Health. Elsevier, Netherlands. DOI: https://doi.org/10.1016/B978-0-12-813148-0.00006-2
Giovanni, D.L., and Francesco, S., 2020. Statistical significance: P Value, 0.05 threshold, and applications to radiomics-reasons for a conservative approach. European Radiology Experimental, 4, p.18. DOI: https://doi.org/10.1186/s41747-020-0145-y
Gochman, E., Mahajna, J., Shenzer, P., Dahan, A., Blatt, A., Elyakim, R., and Reznick, A.Z., 2012. The expression of iNOS and nitrotyrosine in colitis and colon cancer in humans. Acta Histochemica, 114, pp.827-835. DOI: https://doi.org/10.1016/j.acthis.2012.02.004
Gopčević, K.R., Rovčanin, B.R., Tatić, S.B., Krivokapić, Z.V., Gajić, M.M., and Dragutinović, V.V., 2013. Activity of superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase in different stages of colorectal carcinoma. Digestive Diseases and Sciences, 58, pp.2646-2652. DOI: https://doi.org/10.1007/s10620-013-2681-2
Hankey, W., Frankel, W.L., and Groden, J., 2018. Functions of the APC tumor suppressor protein dependent and independent of canonical WNT signaling: Implications for therapeutic targeting. Cancer and Metastasis Reviews, 37, pp.159-172. DOI: https://doi.org/10.1007/s10555-017-9725-6
He, L., He, T., Farrar, S., Ji, L., Liu, T., and Ma, X., 2017. Antioxidants maintain cellular redox homeostasis by elimination of reactive oxygen species. Cellular Physiology and Biochemistry, 44, pp.532-553. DOI: https://doi.org/10.1159/000485089
Hidaka, E., Maeda, C., Nakahara, K., Wakamura, K., Ishiyama, Y., Shimada, S., Seki, J., Takano, Y., Oae, S., and Enami, S.E., 2019. High serum CA19-9 concentration predicts poor prognosis in elderly patients with stage IV Colorectal cancer. Gastrointestinal Tumors, 5, pp.117-124. DOI: https://doi.org/10.1159/000493793
Ibrahem, S., Ahmed, H., and Zangana, S., 2022. Trends in colorectal cancer in Iraq over two decades: Incidence, mortality, topography and morphology. Annals of Saudi Medicine, 42, pp.252-261. DOI: https://doi.org/10.5144/0256-4947.2022.252
Irawan, B., Labeda, I., Lusikooy, R.E., Sampetoding, S., Kusuma, I.M., Uwuratuw, J.A., Syarifuddin, E., Warsinggih, Prihantono, and Faruk, M., 2020. Association of superoxide dismutase enzyme with staging and grade of differentiation colorectal cancer: A cross-sectional study. Annals of Medicine and Surgery (Lond), 58, pp.194-199. DOI: https://doi.org/10.1016/j.amsu.2020.08.032
Irazabal, M.V., and Torres, V.E., 2020. Reactive oxygen species and redox signaling in chronic kidney disease. Cells, 9, p.1342. DOI: https://doi.org/10.3390/cells9061342
Jafri, H.S.M.O., Mushtaq, S., and Baig, S., 2021. Detection of kras gene in colorectal cancer patients through liquid biopsy: A cost-effective method. Journal of College of Physicians and Surgeons Pakistan, 31, pp.1174-1178. DOI: https://doi.org/10.29271/jcpsp.2021.10.1174
Janion, K., Strzelczyk, J.K., Walkiewicz, K.W., Biernacki, K., Copija, A., Szczepańska, E., and Nowakowska-Zajdel, E., 2022. Evaluation of Malondialdehyde level, total oxidant/antioxidant status and oxidative stress index in colorectal cancer patients. Metabolites, 12, p.1118. DOI: https://doi.org/10.3390/metabo12111118
Janion, K., Szczepańska, E., Nowakowska-Zajdel, E., Strzelczyk, J., and Copija, A., 2020a. Selected oxidative stress markers in colorectal cancer patients in relation to primary tumor location-a preliminary research. Medicina (Kaunas), 56, p.47.
Janion, K., Szczepańska, E., Nowakowska-Zajdel, E., Strzelczyk, J., and Copija, A., 2020b. Selected oxidative stress markers in colorectal cancer patients in relation to primary tumor location-a preliminary research. Medicina (Kaunas), 56, p.47. DOI: https://doi.org/10.3390/medicina56020047
Jelic, M.D., Mandic, A.D., Maricic, S.M., and Srdjenovic, B.U., 2021. Oxidative stress and its role in cancer. Journal of Cancer Research and Therapeutics, 17, pp.22-28. DOI: https://doi.org/10.4103/jcrt.JCRT_862_16
Kang, M., Jeong, S., Park, S., Nam, S., Chung, J.W., Kim, K.O., An, J., and Kim, J.H., 2023. Significance of 8-OHdG expression as a predictor of survival in colorectal cancer. Cancers (Basel), 15, p.4613. DOI: https://doi.org/10.3390/cancers15184613
Katona, B.W., and Weiss, J.M., 2020. Chemoprevention of colorectal cancer, Gastroenterology, 158, pp.368-388. DOI: https://doi.org/10.1053/j.gastro.2019.06.047
Kennedy, L., Sandhu, J.K., Harper, M.E., and Cuperlovic-Culf, M., 2020. Role of glutathione in cancer: From mechanisms to therapies. Biomolecules, 10, p.1429. DOI: https://doi.org/10.3390/biom10101429
Kreutzmann, M., Kraus, B.J., Christa, M., Störk, S., Jansen, E.H., Stopper, H., and Schupp, N., 2023. Differential modulation of markers of oxidative stress and DNA damage in arterial hypertension. Antioxidants (Basel), 12, p.1965. DOI: https://doi.org/10.3390/antiox12111965
Lakemeyer, L., Sander, S., Wittau, M., Henne-Bruns, D., Kornmann, M., and Lemke, J., 2021. Diagnostic and prognostic value of CEA and CA19-9 in colorectal cancer. Diseases, 9, p.21. DOI: https://doi.org/10.3390/diseases9010021
Lakemeyer, L.E., 2023. Colorectal Cancer: Impact of CEA and CA19-9.Universität Ulm, Ulm.Lewandowska, A., Rudzki, G., Lewandowski, T., Stryjkowska-Gora, A., and Rudzki, S., 2022. Risk factors for the diagnosis of colorectal cancer. Cancer Control, 29, p.1-15. DOI: https://doi.org/10.1177/10732748211056692
Li, T., Qian, Y., Li, H., and Deng, J., 2018. Combination of serum lipids and cancer antigens as a novel marker for colon cancer diagnosis. Lipids in Health and Disease, 17, p.261. DOI: https://doi.org/10.1186/s12944-018-0911-5
Lorestani, S., Hashemy, S.I., Mojarad, M., Keyvanloo Shahrestanaki, M., Bahari, A., Asadi, M., and Zahedi Avval, F.Z., 2018. Increased glutathione reductase expression and activity in colorectal cancer tissue samples: An investigational study in Mashhad, Iran. Middle East Journal of Cancer, 9, p.99-104.
Luo, H., Fang, Y.J., Lu, M.S., Pan, Z.Z., Huang, J., Chen, Y.M., and Zhang, C.X., 2019. Dietary and serum vitamins A and E and colorectal cancer risk in Chinese population: A case-control study. European Journal of Cancer Prevention, 28, p.268-277. DOI: https://doi.org/10.1097/CEJ.0000000000000452
Lv, H., Zhen, C., Liu, J., Yang, P., Hu, L., and Shang, P., 2019. Unraveling the potential role of glutathione in multiple forms of cell death in cancer therapy.Oxidative Medicine and Cellular Longevity, 2019, p.3150145. DOI: https://doi.org/10.1155/2019/3150145
Malinowska, K., Mik, M., Dziki, Ł., Dziki, A., and Majsterek, I.J., 2015. Evaluation of antioxidant defense in patients with colorectal carcinoma. Polish Journal of Surgery, 87, pp.357-361. DOI: https://doi.org/10.1515/pjs-2015-0069
Marrocco, I., Altieri, F., and Peluso, I., 2017. Measurement and clinical significance of biomarkers of oxidative stress in humans. Oxidative Medicine and Cellular Longevity, 2017, p.6501046. DOI: https://doi.org/10.1155/2017/6501046
Mohan, M., Rafi, S.T.M., Muthusami, S., Muthusami, S., Ramalingam, S., Sambandam, Y., Selvendiran, K., Ramachandran, I., and Kumaran, R.I., 2022. Targeting the metabolism in cancer cells for cancer therapy. In: Handbook of Oxidative Stress in Cancer: Therapeutic Aspects. Springer, Germany. DOI: https://doi.org/10.1007/978-981-16-1247-3_216-1
Najafi, A., Keykhaee, M., Kazemi, M.H., Karimi, M.Y., Khorramdelazad, H., Aghamohamadi, N., Bolouri, M.R., Ghaffari-Nazari, H., Mirsharif, E.S., Karimi, M., Dehghan Manshadi, H.R., Mahdavi, S.R., Safari, E., Jalali, S.A., Falak, R., and Khoobi, M., 2023. Catalase-gold nanoaggregates manipulate the tumor microenvironment and enhance the effect of low-dose radiation therapy by reducing hypoxia. Biomedicine and Pharmacotherapy, 167, p.115557. DOI: https://doi.org/10.1016/j.biopha.2023.115557
Nalkiran, I., Turan, S., Arikan, S., Kahraman, Ö.T., Acar, L., Yaylim, I., and Ergen, A., 2015. Determination of gene expression and serum levels of MnSOD and GPX1 in colorectal cancer. Anticancer Research, 35, pp.255-259.
Patel, M., Mcsorley, S.T., Park, J.H., Roxburgh, C.S.D., Edwards, J., Horgan, P.G., and Mcmillan, D.C., 2018. The relationship between right-sided tumour location, tumour microenvironment, systemic inflammation, adjuvant therapy and survival in patients undergoing surgery for colon and rectal cancer. British Journal of Cancer, 118, pp.705-712. DOI: https://doi.org/10.1038/bjc.2017.441
Raj Rai, S., Bhattacharyya, C., Sarkar, A., Chakraborty, S., Sircar, E., Dutta, S., and Sengupta, R., 2021. Glutathione: Role in oxidative/nitrosative stress, antioxidant defense, and treatments. ChemistrySelect, 6, pp.4566-4590. DOI: https://doi.org/10.1002/slct.202100773
Rašić, I., Rašić, A., Akšamija, G., and Radović, S., 2018. The relationship between serum level of malondialdehyde and progression of colorectal cancer. Acta Clinica Croatica, 57, pp.411-416. DOI: https://doi.org/10.20471/acc.2018.57.03.02
Rasool, M., Malik, A., Waquar, S., Ain, Q.T., Rasool, R., Asif, M., Anfinan, N., Haque, A., Alam, H., Ahmed, S., and Hamid Hamdard, M., 2021. Assessment of clinical variables as predictive markers in the development and progression of colorectal cancer. Bioengineered, 12, pp.2288-2298. DOI: https://doi.org/10.1080/21655979.2021.1933680
Sawicki, T., Ruszkowska, M., Danielewicz, A., Niedźwiedzka, E., Arłukowicz, T., and Przybyłowicz, K.E., 2021. A review of colorectal cancer in terms of epidemiology, risk factors, development, symptoms and diagnosis. Cancers (Basel), 13, p.2025. DOI: https://doi.org/10.3390/cancers13092025
Wang, X., O‘Connell, K., Jeon, J., Song, M., Hunter, D., Hoffmeister, M., Lin, Y., Berndt, S., Brenner, H., Chan, A.T., Chang-Claude, J., Gong, J., Gunter, M.J., Harrison, T.A., Hayes, R.B., Joshi, A., Newcomb, P., Schoen, R., Slattery, M.L., Vargas, A., Potter, J.D., Le Marchand, L., Giovannucci, E., White, E., Hsu, L., Peters, U., and Du, M., 2019. Combined effect of modifiable and non-modifiable risk factors for colorectal cancer risk in a pooled analysis of 11 population-based studies. BMJ Open Gastroenterology, 6, p.e000339. DOI: https://doi.org/10.1136/bmjgast-2019-000339
Wang, Y., Qi, H., Liu, Y., Duan, C., Liu, X., Xia, T., Chen, D., Piao, H.L., and Liu, H.X., 2021. The double-edged roles of ROS in cancer prevention and therapy. Theranostics, 11, p.4839. DOI: https://doi.org/10.7150/thno.56747
Wei, R., Qiu, H., Xu, J., Mo, J., Liu, Y., Gui, Y., Huang, G., Zhang, S., Yao, H., Huang, X., and Gan, Z., 2020. Expression and prognostic potential of GPX1 in human cancers based on data mining. Annals of Translational Medicine, 8, p.124. DOI: https://doi.org/10.21037/atm.2020.02.36
Wong, M.C., Ding, H., Wang, J., Chan, P.S., and Huang, J., 2019. Prevalence and risk factors of colorectal cancer in Asia. Intestinal Research, 17, pp.317-329. DOI: https://doi.org/10.5217/ir.2019.00021
Zhao, Y., Wang, H., Zhou, J., and Shao, Q.J.C., 2022. Glutathione peroxidase GPX1 and its dichotomous roles in cancer. Cancers (Basel), 14, p.2560. DOI: https://doi.org/10.3390/cancers14102560
Zhu, G., Pei, L., Xia, H., Tang, Q., and Bi, F., 2021. Role of oncogenic KRAS in the prognosis, diagnosis and treatment of colorectal cancer. Molecular Cancer, 20, pp.1-17. DOI: https://doi.org/10.1186/s12943-021-01441-4
Zińczuk, J., Maciejczyk, M., Zaręba, K., Pryczynicz, A., Dymicka-Piekarska, V., Kamińska, J., Koper-Lenkiewicz, O., Matowicka-Karna, J., Kędra, B., Zalewska, A.J.C., and Guzińska-Ustymowicz K., 2020. Pro-oxidant enzymes, redox balance and oxidative damage to proteins, lipids and DNA in colorectal cancer tissue. Is oxidative stress dependent on tumour budding and inflammatory infiltration? Cancers (Basel), 12, p.1636. DOI: https://doi.org/10.3390/cancers12061636
Zińczuk, J., Maciejczyk, M., Zaręba, K., Romaniuk, W., Markowski, A., Kędra, B., Zalewska, A., Pryczynicz, A., Matowicka-Karna, J., and Guzińska-Ustymowicz, K., 2019a. Antioxidant barrier, redox status, and oxidative damage to biomolecules in patients with colorectal cancer. Can malondialdehyde and catalase be markers of colorectal cancer advancement? Biomolecules, 9, p.637.
Zińczuk, J., Maciejczyk, M., Zaręba, K., Romaniuk, W., Markowski, A., Kędra, B., Zalewska, A., Pryczynicz, A., Matowicka-Karna, J., and Guzińska-Ustymowicz, K., 2019b. Antioxidant barrier, redox status, and oxidative damage to biomolecules in patients with colorectal cancer. Can malondialdehyde and catalase be markers of colorectal cancer advancement? Biomolecules, 9, p.637. DOI: https://doi.org/10.3390/biom9100637
Zińczuk, J., Zaręba, K., Kamińska, J., Koper-Lenkiewicz, O.M., Dymicka-Piekarska, V., Pryczynicz, A., Guzińska-Ustymowicz, K., Kędra, B., Matowicka-Karna, J., Żendzian-Piotrowska, M., Zalewska, A., and Maciejczyk, M., 2021. Association of tumour microenvironment with protein glycooxidation, DNA damage, and nitrosative stress in colorectal cancer. Cancer Management and Research, 13, pp.6329-6348. DOI: https://doi.org/10.2147/CMAR.S314940
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